NDLI: Oxygen availability regulates metabolism and gene expression in trout hepatocyte cultures.

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Oxygen availability regulates metabolism and gene expression in trout hepatocyte cultures.

Content Provider	Semantic Scholar
Author	Rissanen, Eeva Tranberg, Hanna K. Nikinmaa, Mikko
Copyright Year	2006
Abstract	We studied the metabolic rate, cellular energetic state, hypoxia-inducible factor-1 (HIF-1) activation, and expression of enzymes involved in energy metabolism using rainbow trout (Oncorhynchus mykiss) hepatocytes over the oxygen range from 21 to 1 kPa. Oxygen dependence of these factors was assessed by gradually reducing oxygen supply to cells from 21 kPa to 10, 5, 2, and 1 kPa. Moreover, time course experiments for up to 20 h at oxygen tensions of 1 and 2 kPa were carried out. Reduction of oxygen from 21 kPa to 10, 5, 2, and 1 kPa decreased metabolic rate of the cells by 14, 24, 37, and 46%, respectively. This response was instantaneous and fully reversible upon reoxygenation. Cellular ATP content and the expression of all mRNAs studied decreased when oxygen was reduced from 21 to 5 and 2 kPa. The lowest ATP levels, approximately 43% of the initial value, were measured at 5 kPa of oxygen, whereas the reduction in mRNA amounts was most pronounced at 2 kPa. At 1 kPa oxygen tension, both ATP content and mRNA amounts returned to normoxic (21 kPa) levels with a concomitant activation of HIF-1, indicating reorganization of energy metabolism in adaptation of cells to low oxygen supply. These results show that oxygen has a direct regulatory effect on metabolism of trout hepatocyte cultures, supporting the view that oxygen has a profound role in metabolic regulation in cells.
Starting Page	279
Ending Page	287
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1152/ajpregu.00025.2006
PubMed reference number	16778071
Journal	Medline
Volume Number	291
Issue Number	5
Alternate Webpage(s)	http://ajpregu.physiology.org/content/ajpregu/291/5/R1507.full.pdf
Alternate Webpage(s)	https://doi.org/10.1152/ajpregu.00025.2006
Journal	American journal of physiology. Regulatory, integrative and comparative physiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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