NDLI: Infectious and non-infectious mutants of cauliflower mosaic virus DNA.

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Infectious and non-infectious mutants of cauliflower mosaic virus DNA.

Content Provider	Semantic Scholar
Author	Melcher, U. Steffens, David L. Lyttle, D. J. Lebeurier, Geneviève Lin, Haoran Essenberg, Richard C.
Copyright Year	1986
Abstract	Mutants of cauliflower mosaic virus (CaMV), generated in vitro by modification of recombinant DNA plasmids containing the viral genome, either retained the ability to induce disease symptoms on turnip plants, produced less severe symptoms or failed to induce symptoms. Wild-type symptoms were produced by a variant CaMV DNA of the Cabbage S isolate that had 4 bp in open reading frame (ORF) III replaced with a 16 bp sequence. Less severe symptoms, due to a delay in symptom appearance relative to inoculation with wild-type DNA, were induced by a mutant with a frameshift mutation in ORF II (pSA103). CaMV DNA, recovered from plants infected with pSA103, contained a second mutation which restored the original translation reading frame. Nucleic acid hybridization to 'squishes' of leaf tissue from plants that had been inoculated with mutant DNAs that included DNAs modified in each of the six major ORFs of CaMV DNA revealed that only those plants that appeared diseased had detectable CaMV nucleic acid in uninoculated leaves. Replicated CaMV DNA was also not detected in non-encapsidated and virion DNA fractions from inoculated leaves of non-diseased plants.
Starting Page	28
Ending Page	34
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/67/7/JV0670071491.pdf?accname=guest&checksum=8B1BDF3CF7A53585412E8CC5A2EF4B6B&expires=1539664719&id=id
PubMed reference number	3723112v1
Volume Number	67
Part	7
Journal	The Journal of general virology
Language	English
Access Restriction	Open
Subject Keyword	Cauliflower Mosaic Virus Cell Nucleus Contain (action) DNA Fingerprinting Frameshift Mutation function Mosaic Organism Mosaic Viruses Nucleic Acid Hybridization Nucleic Acids Open Reading Frames Plasmids Reading Frames (Nucleotide Sequence) Recombinant DNA Recombinants Thioctic Acid Turnip - dietary mutant viral genome location
Content Type	Text
Resource Type	Article

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