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Identi fi cation of Suppressors of mbk-2 / DYRK by Whole-Genome Sequencing
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Yuemeng Wang, Jennifer T. Rasoloson, Dominique Stitzel, Michael L. O. Kevin F. Connell Smith, Harold E. Seydoux, Geraldine |
| Copyright Year | 2014 |
| Abstract | Screening for suppressor mutations is a powerful method to isolate genes that function in a common pathway or process. Because suppressor mutations often do not have phenotypes on their own, cloning of suppressor loci can be challenging. A method combining whole-genome sequencing (WGS) and single nucleotide polymorphism (SNP) mapping (WGS/SNP mapping) was developed to identify mutations with visible phenotypes in C. elegans. We show here that WGS/SNP mapping is an efficient method to map suppressor mutations without the need for previous phenotypic characterization. Using RNA-mediated interference to test candidate loci identified by WGS/SNP mapping, we identified 10 extragenic and six intragenic suppressors of mbk-2, a DYRK family kinase required for the transition from oocyte to zygote. Remarkably, seven suppressors are mutations in cell-cycle regulators that extend the timing of the oocyteto-zygote transition. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.g3journal.org/content/ggg/4/2/231.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
