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Effective heat inactivation of Mycobacterium avium subspecies paratuberculosis in milk
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hoolwerf Giffel, Te |
| Copyright Year | 2006 |
| Abstract | This study was designed to evaluate the effectiveness of high-temperature short-time (HTST) pasteurization and homogenization on the inactivation of Mycobacterium avium subsp. paratuberculosis (MAP). MAP-contaminated feces of cows with clinical symptoms of Johne’s disease was used to contaminate the raw milk with MAP to final concentrations varying between 10 to 3.5 * 10 cells per ml. Twenty two different time-temperature combinations were evaluated ranging from 60 90°C and holding times of 6, 10 and 15 seconds using a pilot scale turbulent-flow pasteurizer. A total of 136 observations were obtained; prior homogenization occurred in 34 of these samples. Regression analysis of 69 data points allowed for modeling of the heat inactivation of MAP. From these inactivation experiments the following conclusions can be drawn: HTST pasteurization conditions as applied in the Dutch dairy industry result in a >6 log reduction of MAP. Homogenization did not contribute significantly to the inactivation. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.paratuberculosis.info/web/images/stories/pdfs/157 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |