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Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Canezin, J. Cailleux, Annie Turcant, Alain Bouil, Anne Le Harry, Patrick Allain, Patrick |
| Copyright Year | 2001 |
| Abstract | A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step liquid-liquid extraction on 1 ml blood or urine was used. The lower limit for quantitative determination was 0.02 microg/l for LSD and iso-LSD. The analytical procedure has been applied in two positive cases (case 1: LSD=0.31 microg/l, iso-LSD=0.27 microg/l in plasma and LSD=1.30 microg/l, iso-LSD=0.82 microg/l in urine; case 2: LSD=0.24 microg/l, iso-LSD=0.6 microg/l in urine). LSD metabolism was investigated using MS-MS neutral loss monitoring for the screening of potential metabolites. The main metabolite was 2-oxo-3-hydroxy-LSD (O-H-LSD) present in urine at the concentrations of 2.5 microg/l and 6.6 microg/l, respectively, for case 1 and 2, and was not present in plasma. Nor-LSD was also found in urine at 0.15 and 0.01 microg/l levels. Nor-iso-LSD, lysergic acid ethylamide (LAE), trioxylated-LSD, lysergic acid ethyl-2-hydroxyethylamide (LEO) and 13 and 14-hydroxy-LSD and their glucuronide conjugates were detected in urine using specific MS-MS transitions. |
| Starting Page | 15 |
| Ending Page | 27 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0378-4347(01)00386-3 |
| PubMed reference number | 11817305 |
| Journal | Medline |
| Volume Number | 765 |
| Issue Number | 1 |
| Alternate Webpage(s) | https://www.erowid.org/references/texts/show/6266docid6184 |
| Alternate Webpage(s) | https://doi.org/10.1016/S0378-4347%2801%2900386-3 |
| Journal | Journal of chromatography. B, Biomedical sciences and applications |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
