NDLI: E4BP4 is a cardiac survival factor and essential for embryonic heart development

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E4BP4 is a cardiac survival factor and essential for embryonic heart development

Content Provider	Semantic Scholar
Author	Weng, Yi-Jiun Hsieh, Dennis Jine-Yuan Kuo, Wei-Wen Lai, Tung-Yuan Hsu, Hsi-Hsien Tsai, Chang-Hai Tsai, Fuu-Jen Lin, Ding-Yu Lin, James A. Huang, Chih-Yang Tung, Kwong-Chung
Copyright Year	2010
Abstract	The bZIP transcription factor E4BP4, has been demonstrated to be a survival factor in pro-B lymphocytes. GATA factors play important roles in transducing the IL-3 survival signal and transactivating the downstream survival gene, E4BP4. In heart, GATA sites are essential for proper transcription of several cardiac genes, and GATA-4 is a mediator of cardiomyocyte survival. However, the role E4BP4 plays in heart is still poorly understood. In this study, Dot-blot hybridization assays using Dig-labeled RNA probes revealed that the E4BP4 gene was expressed in cardiac tissue from several species including, monkey, dog, rabbit, and human. Western blot analysis showed that the E4BP4 protein was consistently present in all of these four species. Furthermore, immunohistochemistry revealed that the E4BP4 protein was overexpressed in diseased heart tissue in comparison with normal heart tissue. In addition, the overexpression of E4BP4 in vitro activated cell survival signaling pathway of cardiomyocytes. At last, siRNA-mediated knock down of E4BP4 in zebrafish resulted in malformed looping of the embryonic heart tube and decreased heart beating. Based on these results, we conclude that E4BP4 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.
Starting Page	187
Ending Page	194
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1007/s11010-010-0417-6
PubMed reference number	20186462
Journal	Medline
Volume Number	340
Alternate Webpage(s)	http://ir.cmu.edu.tw/ir/bitstream/310903500/36806/1/20100903170725.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s11010-010-0417-6
Journal	Molecular and Cellular Biochemistry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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