NDLI: Resfriamento de embriões de tambaqui (Colossoma macropomum) em diferentes tempos de estocagem

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Resfriamento de embriões de tambaqui (Colossoma macropomum) em diferentes tempos de estocagem

Content Provider	Semantic Scholar
Author	Pessoa, Nathalie Ommundsen Evangelista, Janaína Serra Azul Monteiro Filho, Francisco Gerson Mendes De Sousa Sousa, Míriam Luiza Nogueira Martins De Lourenço, Athur Vinícius Sampaio, C. M. S.
Copyright Year	2013
Abstract	The technique of cryopreservation of fish embryos has not efficient results yet, however, the cooling of embryos has been used with satisfactory results. The aim of this study was cooling embryos Colossoma macropomum subjected to a temperature of 2oC at different times of storage. Embryos were used in the following embryonic stages: blastoderm - 1.2 hours post-fertilization (hpf), epibolia - 5-hpf; closing blastopore - 8-hpf; appearance of the optic vesicle - 13 hpf. One hundred embryos, of each stage of ontogeny selected, were treated with glucose 0.5 M solution with 10% methanol, glucose 0.5 M with 10% methyl glycol and glucose 0.5 M in dimethylsulfoxide (DMSO) and cooled at 2°C for 6, 8, 10 and 12 hours. Statistical analysis was performed by ANOVA and Tukey test with 5% significance. We observed a significantly higher difference in complete development of live larvae with the group treated with methanol solution of glucose and a greater survival in the embryos at earlier developmental stages (8-hpf and 13-hpf). It is concluded, therefore, that the embryonic stages of closing blastopore (8-hpf) and appearance of the optic vesicle (13 hpf) can be considered the most recommended for use in cooling protocol of embryos of C. macropomum maintained for 8 hours at 2°C. Key words : Aquaculture, fish of South America, embryonic development, cryoprotectants, larval survival, storage.
Starting Page	323
Ending Page	344
Page Count	22
File Format	PDF HTM / HTML
DOI	10.5935/rbhsa.v7i2.88
Volume Number	7
Alternate Webpage(s)	http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/download/88/2104
Alternate Webpage(s)	https://doi.org/10.5935/rbhsa.v7i2.88
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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