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In vivo digestive stability of soybean β-conglycinin β-subunit in WZS minipigs.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Huang, Qiong Xu, Hai Bin Yu, Zhou Lu Liu, Shan Gao, Peng |
| Copyright Year | 2015 |
| Abstract | By now, the digestive stability experiments provided by most authoritative organizations are in vitro tests. Evaluating the protein digestive stability with in vivo models should be more objective. The present study aimed to verify the in vivo digestibility of soybean β-conglycinin β-subunit in Wuzhishan (WZS) minipigs. Three minipigs were surgically fitted with O-stomach and T-ileum cannulae and fed with soybean meals. According to SDS-PAGE, the 50 kD fraction of soybean β-conglycinin β-subunit persisted in the gastric fluid until 6 h after feeding, which was detected at 3 h and clearly visible at 4-6 h in the intestinal fluid. Western blot with anti-β-conglycinin β-subunit McAb confirmed it. |
| Starting Page | 40 |
| Ending Page | 41 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.besjournal.com/Articles/Archive/2015/No1/201501/P020150107340239849934.pdf |
| PubMed reference number | 25566867v1 |
| Alternate Webpage(s) | https://doi.org/10.3967/bes2015.010 |
| DOI | 10.3967/bes2015.010 |
| Journal | Biomedical and environmental sciences : BES |
| Volume Number | 28 |
| Issue Number | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cannula device Digestion Gastric Tissue Operative Surgical Procedures Polyacrylamide Gel Electrophoresis SDS-PAGE Seizures Soybean Oil Soybeans Stomach Neoplasms Swine, Miniature Tritium Trypsin Inhibitor, Kunitz Soybean Western Blotting |
| Content Type | Text |
| Resource Type | Article |