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A Titrimetric Method for the Determination of Sodium Pregnanediol Glucuronidate in the Urine of Pregnant Women
| Content Provider | Semantic Scholar |
|---|---|
| Author | Allen, Willard M. Viergiver, Ellenmae |
| Copyright Year | 1941 |
| Abstract | Studies of the metabolism of the sex hormones have been based for the most part on the determination of these compounds in the blood and urine. The estrogens and androgens are present in sufficient amounts so that they can be measured by bioassay but so little progesterone is present either in blood or urine that it is virtually impossible to detect it. A closely related compound, pregnanediol, is found in the urine but this is biologically inactive, so that its presence cannot be detected by bioassay. The compound must, therefore, be determined by some gravimetric, colorimetric, or other chemical procedure. The first procedure devised and the one most widely used is that of Venning (1, 2). In this procedure the compound is isolated as the sodium salt of pregnanediol glucuronide and the amount present determined by weighing. A second method has also been devised by Astwood and Jones (3). In this procedure the urine is hydrolyzed by strong acid to deconjugate the sodium pregnanediol glucuronidate and thereby liberate the pregnanediol. In this case pregnanediol rather than sodium pregnanediol glucuronidate is determined by weighing. Both of these methods require the isolation of an essentially pure compound before the final gravimetric procedure can be employed. |
| Starting Page | 837 |
| Ending Page | 852 |
| Page Count | 16 |
| File Format | PDF HTM / HTML |
| Volume Number | 141 |
| Alternate Webpage(s) | http://www.jbc.org/content/141/3/837.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
