NDLI: Carbon Flux Analysis in a Pantothenate Overproducing Corynebacterium Glutamicum Strain

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Carbon Flux Analysis in a Pantothenate Overproducing Corynebacterium Glutamicum Strain

Content Provider	Semantic Scholar
Author	Chassagnole, Christophe Létisse, Fabien Diano, Audrey Lindley, Nic D.
Copyright Year	2004
Abstract	Carbon flux analysis during a pseudo-stationary phase of metabolite accumulation in a genetically engineered strain of Corynebacterium glutamicum, containing plasmids leading to over-expression of the ilvBNCD and panBC operons, has identified the basic metabolic constraints governing the potential of this bacterium to produce pantothenate. Carbon flux converging on pyruvate (75% of glucose uptake) is controlled by anabolic precursor requirements and NADPH demand provoking high carbon loss as CO2 via the pentose pathway. Virtually all the flux of pyruvate is directed into the branched pathway leading to both valine and pantothenate production, but flux towards valine is tenfold higher than that transformed to pantothenate, indicating that significant improvements will only be obtained if carbon flux at the ketoisovalerate branchpoint can be modulated.
Starting Page	129
Ending Page	134
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1023/A:1020353124066
Alternate Webpage(s)	http://www.rmsb.u-bordeaux2.fr/BTK/abstracts/26-Chassagnole.pdf
PubMed reference number	12241042
Alternate Webpage(s)	https://doi.org/10.1023/A%3A1020353124066
Journal	Medline
Volume Number	29
Journal	Molecular Biology Reports
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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