NDLI: Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid.

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Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid.

Content Provider	Semantic Scholar
Author	Hiszczyńska-Sawicka, Elżbieta Kur, Józef W.
Copyright Year	1995
Abstract	The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid orip15A region. IHF binds to the single site of the DNA fragment containing the orip15A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the orip15A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81, 1-15. One ihf binding site was also found in the oriColE1 region sequence with three mismatches in relation to this consensus sequence.
File Format	PDF HTM / HTML
DOI	10.18388/abp.1995_4675
Alternate Webpage(s)	https://ojs.ptbioch.edu.pl/index.php/abp/article/download/4675/3728
Alternate Webpage(s)	http://www.actabp.pl/pdf/1_1995/103.pdf
PubMed reference number	7653150
Alternate Webpage(s)	https://doi.org/10.18388/abp.1995_4675
Journal	Medline
Volume Number	42
Issue Number	1
Journal	Acta biochimica Polonica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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