NDLI: Ethanol Mediates Cell Cycle Arrest and Apoptosis in SK-N-SH Neuroblastoma Cells

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Ethanol Mediates Cell Cycle Arrest and Apoptosis in SK-N-SH Neuroblastoma Cells

Content Provider	Semantic Scholar
Author	Lee, Maria Song, Byoung-Joon Kwon, Yongil
Copyright Year	2014
Abstract	BACKGROUND The mechanisms of cell or organ damage by chronic alcohol consumption are still poorly understood. The present study aimed to investigate the role of the mitogen-activated protein kinases during ethanol-induced damage to SK-N-SH neuroblastoma cells. METHODS Cells were treated with ethanol and subsequently analyzed for cell morphology, viability, and DNA fragmentation. Immunoblot analysis was performed to assess various proteins levels associated with cell cycle arrest and apoptosis after ethanol exposure. RESULTS Ethanol induced time- and dose-dependent cell death in SK-N-SH cells and increased c-Jun N-terminal protein kinase (JNK) activity in a time- and concentration dependent manner. In contrast, p38 kinase activity increased transiently. After treatment with JNK or p38 kinase inhibitors, ethanol-induced cell death significantly reduced. Ethanol-induced cell death was accompanied by increased cytochrome c release and caspase 3 activity observed at 12 h. In contrast, the level of anti-apoptotic Bcl-2 protein did not change. Ethanol also increased the phosphorylation of p53 and p53 activation was followed by an increase in the p21 tumor suppressor protein accompanied by a gradual decrease in phospho-Rb protein. CONCLUSION Our results suggest that ethanol mediates apoptosis of neuroblastoma cells by stimulating p53-related cell cycle arrest mediated through activation of the JNK-related pathway.
Starting Page	39
Ending Page	46
Page Count	8
File Format	PDF HTM / HTML
DOI	10.15430/jcp.2014.19.1.39
PubMed reference number	25337571
Journal	Medline
Volume Number	19
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/2d/d6/jcp-20-164.PMC4492362.pdf
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7d/30/jcp-19-039.PMC4189479.pdf
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2d/d6/jcp-20-164.PMC4492362.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/7d/30/jcp-19-039.PMC4189479.pdf
Alternate Webpage(s)	https://doi.org/10.15430/jcp.2014.19.1.39
Journal	Journal of cancer prevention
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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