NDLI: Nonselective cation conductance activated by muscarinic and purinergic receptors in rat spiral ganglion neurons.

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Nonselective cation conductance activated by muscarinic and purinergic receptors in rat spiral ganglion neurons.

Content Provider	Semantic Scholar
Author	Ito, Ken Kenneth Dulon, Didier
Copyright Year	2002
Abstract	The present study characterizes the ionic conductances activated by acetylcholine (ACh) and ATP, two candidate neuromodulators, in isolated spiral ganglion neurons (SGNs). Brief application (1 s) of ACh evoked in a dose-dependent manner (EC(50) = 4.1 microM) a reversible inward current with a long latency (average 1.3 s), at holding potential (V(h)) = -50 mV. This current was reversibly blocked by atropine and mimicked by muscarine. Application of ATP also evoked a reversible inward current at V(h) = -50 mV, but the current showed two components. A fast component with a short latency was largely reduced when N-methyl-D-glucamine (NMDG) replaced extracellular sodium, implying a P2X-like ionotropic conductance. The second component had a longer latency (average 1.1 s) and was presumably activated by metabotropic P2Y-like receptors. The second component of ATP-evoked current shared similar characteristics with the responses evoked by ACh: the current reversed near 0 mV, displayed inward rectification, could be carried by NMDG, and was insensitive to extracellular and intracellular calcium. This ACh-/ATP-evoked conductance was reversibly inhibited by preapplication of ionomycin. These results suggest that muscarinic receptors and purinergic metabotropic receptors activate a similar large nonselective cation conductance via a common intracellular pathway in SGNs, a candidate mechanism to regulate neuronal excitability of SGNs.
Starting Page	323
Ending Page	333
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1152/ajpcell.00364.2001
PubMed reference number	11940528
Journal	Medline
Volume Number	282
Issue Number	5
Alternate Webpage(s)	http://ajpcell.physiology.org/content/ajpcell/282/5/C1121.full.pdf
Alternate Webpage(s)	https://doi.org/10.1152/ajpcell.00364.2001
Journal	American journal of physiology. Cell physiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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