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Effect of osmopriming on germination in seeds of three winter canola ( Brassica napus L . ) varieties under salinity stress
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sanavy, Seyed Ali Mohammad Modarres Ehsanfar, Somayeh |
| Copyright Year | 2009 |
| Abstract | Effects of salinity (in four levels) and priming (in eleven levels with control) on germination and growth parameters of three canola varieties (SLM046, Okapi and Licord) were investigated under laboratory (first experiment) and greenhouse (second experiment) conditions with three replications. The statistical design was factorial laid out in complete randomized design and randomized complete block design in the first and second experiment respectively. At first, seeds were primed in two times (12 and 24 hours) and five osmotic potentials by polyethylenglycol (-10, -12, -14, -16 and –18 bar) in petridishes. At the end of priming, seeds were washed by distilled water, and used for germination test in laboratory and greenhouse conditions. Germination tests of control and primed seeds were conducted in four levels of salinity (0, 6, 12 and 18 ds/m). After planting the seeds, counting germinated seeds was done daily for one week in the first experiment and two weeks in the second one and germination percentage and index and coefficient of velocity were calculated. Other parameters, include length and dry weight of stem and radicle (in first examination) and leaf area, chlorophyll content, plant dry weight, leaf area and leaf weight ratio, specific leaf area and vigour index (in second examination) were measured. Salinity reduced germination percentage and index, coefficient of velocity and vigour index at early stages of canola growth, at normal and salinity stress in laboratory and greenhouse conditions. In laboratory condition, salinity at the level of 6 ds/m increased stem, radicle and seedling dry weight, stem and radicle length, but these parameters were reduced by higher level of salinity, and they became lower than control treatment. Also in greenhouse condition, salinity decreased plant dry weight, leaf area, chlorophyll content, leaf area and weight ratio and specific leaf area. In laboratory conditions, priming in 24 hours increased germination percentage, but this parameter was decreased by 12 hours priming. Priming treatment reduced germination index, stem, radicle and seedling dry weight, stem length and vigour index and increased coefficient of velocity and radicle length in compare with control. In greenhouse conditions, priming increased the coefficient of velocity, chlorophyll content, leaf area, leaf area and leaf weight ratio (in 24 hours priming) and specific leaf area, and decreased emergence percentage and index, leaf weight ratio (in 12 hours priming) and vigour index. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://gcirc.org/fileadmin/documents/Proceedings/IRCWuhan2007vol2/455-457.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
