Loading...
Please wait, while we are loading the content...
Similar Documents
The Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nicholson, Wayne |
| Copyright Year | 2008 |
| Abstract | Bacillus subtilis is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), has remained unknown. A search of the B. subtilis genome database with the amino acid sequences of functional AR/BDHs from Saccharomyces cerevisiae and Bacillus cereus resulted in the identification of a highly similar protein encoded by the B. subtilis ydjL gene. A knockout strain carrying a ydjL::cat insertion mutation was constructed, which (i) abolished 2,3-butanediol production in early stationary phase, (ii) produced no detectable AR or BDH activity in vitro, and (iii) accumulated the precursor acetoin in early stationary phase. The ydjL::cat mutation also affected the kinetics of lactate but not acetate production during stationary-phase cultivation with glucose under oxygen limitation. A very small amount of 2,3-butanediol was detected in very-late-stationary-phase (96-hour) cultures of the ydjL::cat mutant, suggesting the existence of a second gene encoding a minor AR activity. From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/AEM.00881-08 |
| PubMed reference number | 18820069 |
| Journal | Medline |
| Volume Number | 74 |
| Issue Number | 22 |
| Alternate Webpage(s) | http://aem.asm.org/content/74/22/6832.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/AEM.00881-08 |
| Journal | Applied and environmental microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
