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Production of mycelial biomass and exo-polymer by Hericium erinaceus CZ-2: Optimization of nutrients levels using response surface methodology
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cui, Fengjie Liu, Zhi-Qiang Ping, Lifeng Ping, Liying Zhang, Zhicai Dong, Ying Huang |
| Copyright Year | 2010 |
| Abstract | The Doehlert experimental design was used to optimize the production of mycelial biomass and exopolymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract, and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose; 1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour; 2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O. The upscale production was also investigated using a 15 L fermentor using the optimized medium. |
| Starting Page | 299 |
| Ending Page | 307 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s12257-009-0117-9 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s12257-009-0117-9 |
| Alternate Webpage(s) | https://doi.org/10.1007/s12257-009-0117-9 |
| Volume Number | 15 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |