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Isolation and Characterization of a Polycyclic Aromatic hydrocarbons-degrading Enzyme from Xylaria regalis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chang, Shenq-Chyi Chen, Ming-Kai Wei, Ding-Ling Cheng, Min-Kuei |
| Copyright Year | 2007 |
| Abstract | Twenty-one fungal species were screened for capability of removing pyrene and benzo (a) pyrene in culture. Xylaria regalis was found to show the highest activity. An PAHs-degrading enzyme was purified to homogeneity from the culture mycelium of X. regalis. The crude enzyme was subjected to a series of purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interaction phenyl 650M column and Fractogel HW-55 column chromatography, respectively. The enzyme was purified 70.5 fold, giving a 45% yield. The specific activity was 4690U/mg. The molecular mass of the purified PAHsdegrading enzyme estimated by SDS-PAGE was approximately 29kDa. The optimal pH of the purified enzyme was 8.5 and the enzyme was stable between pH 8.0-9.0. The optimal temperature of the enzyme was 30℃ and it's activity was still stable at higher temperatures. The enzyme was found stable within 2h at 100℃ and retained 60% of activity after 8 h incubation. |
| Starting Page | 25 |
| Ending Page | 33 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.7099/FS.200706.0025 |
| Volume Number | 22 |
| Alternate Webpage(s) | http://web2.nmns.edu.tw/TFungi/chinese/data_all/chinese_pdf/chinese2212/2212-4.pdf |
| Alternate Webpage(s) | https://doi.org/10.7099/FS.200706.0025 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
