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Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Balzan, Rena Agius, Dominique Bannister, William H. |
| Copyright Year | 1999 |
| Abstract | Superoxide dismutase (SOD) is considered to be the first line of defense against oxygen toxicity. It exists as a family of three metalloproteins with copper,zinc (Cu,ZnSOD), manganese (MnSOD), and iron (FeSOD) forms. In this work, we have targeted Escherichia coli FeSOD to the mitochondrial intermembrane space (IMS) of yeast cells deficient in mitochondrial MnSOD. Our results show that FeSOD in the IMS increases the growth rate of the cells growing in minimal medium in air but does not protect the MnSOD-deficient yeast cells when exposed to induced oxidative stress. Cloned FeSOD must be targeted to the mitochondrial matrix to protect the cells from both physiological and induced oxidative stress. This confirms that the superoxide radical is mainly generated on the matrix side of the inner mitochondrial membrane of yeast cells, without excluding its potential appearance in the mitochondrial IMS where its elimination by SOD is beneficial to the cells. |
| Starting Page | 144 |
| Ending Page | 147 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/153556633.pdf |
| PubMed reference number | 10066423v1 |
| Volume Number | 256 |
| Issue Number | 1 |
| Journal | Biochemical and biophysical research communications |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adverse reaction to drug Clone Cells Excretory function Inner mitochondrial membrane Intermembranous space of mitochondrion Metalloproteins Mitochondrial Diseases Mitochondrial Inheritance Mitochondrial Membranes Mitochondrial matrix Oxidative Stress Oxygen SOD2 protein, human Superoxide Dismutase Superoxides downregulation of ferrisuperoxide dismutase activity |
| Content Type | Text |
| Resource Type | Article |
