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Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Walter, Gero Scheidtmann, Karl Heinz Carbone, A. Laudano, Andrew P. Doolittle, Russell F. |
| Copyright Year | 1980 |
| Abstract | Antibodies specific for the amino- and carboxy-terminal portions of simian virus 40 large tumor (T) antigen were obtained by immunization of rabbits with synthetic peptides corresponding to these regions. The amino-terminal synthetic peptide has the sequence Ac-Met-Asp-Lys-Val-Leu-Asn-Arg-(Tyr). The tyrosine residue was introduced in order to couple the peptide to bovine serum albumin with bis-diazotized benzidine. The carboxy-terminal peptide has the sequence Lys-Pro-Pro-Thr-Pro-Pro-Pro-Glu-Pro-Glu-Thr. It was coupled to bovine serum albumin with glutaraldehyde. The antisera against both peptides reacted with large T antigen. The specificity of the immune reaction was demonstrated by inhibition experiments using excess synthetic peptides. Furthermore, fragments of T antigen encoded by the nondefective adenovirus 2-simian virus 40 hybrid viruses Ad2+ND2 and Ad2+ND4, which contain the carboxy terminus and lack the amino terminus of large T antigen, were precipitated only with antiserum to the carboxy-terminal peptide. Small T antigen was not precipitated with either serum, suggesting that the amino terminus of small T antigen has a conformation different from that of large T antigen or that it is sterically hindered by a host protein. The procedures used here are of general importance for identification and characterization of gene product. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.77.9.5197 |
| Alternate Webpage(s) | http://www.pnas.org/content/77/9/5197.full.pdf |
| PubMed reference number | 6254066 |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.77.9.5197 |
| Journal | Medline |
| Volume Number | 77 |
| Issue Number | 9 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
