Enhanced Butanol Production by Mutant Strains of Clostridium acetobutylicum in Molasses Medium

Content Provider	Semantic Scholar
Author	Syed, Quratulain Nadeem, Muhammad Nelofer, Rubina
Copyright Year	2008
Abstract	The present study deals with the enhancement of n-butanol production by a mutant strain of Clostridium acetobutylicum using blackstrap molasses as substrate (a by product of sugar industries). The bacteria were cultured in 14 L glass stainless steel fermentor. The parent strain Cl. acetobutylicum PTCC-23 was mutagenized for the improved production of butanol by UV exposure, N-methyl-N-nitro-N-nitrosoguanidine and ethyl methane sulphonate treatments. After extensive screening and optimization, a total of 15 different mutants were selected. The best butanol producing strain was designated as MEMS-7, which yielded 20 % more butanol compared to parent strain after 91 h of incubation at 30 o C. The optimum sugar level in molasses medium was 60.0 g/L. The growth of the parent strain PTCC-23 was inhibited at 1.6 mol/L butanol. The butanol tolerance of the mutant strain MEMS-7 on the other hand was found to be 3.84 mol/L. The fermentation profile and kinetics study indicate the superiority of the mutant strain for n-butanol production from blackstrap molasses.
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Language	English
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Content Type	Text
Resource Type	Article