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Efeitos da administração de uma fração antioxidante de Schinus terebinthifolius sobre parâmetros nociceptivos e de estresse oxidativo em sistema nervoso central de ratos submetidos a um modelo de dor neuropática
| Content Provider | Semantic Scholar |
|---|---|
| Author | Scheid, T. |
| Copyright Year | 2014 |
| Abstract | The management of neuropathic pain, a clinical condition that results of somatosensory system injury, is still a major challenge to clinicians. The implication of reactive oxygen species in the development of this pain type points to antioxidant molecules as new approaches in the management of pain. Schinus terebinthifolius is traditionally used to treat wounds and painful conditions and it was related that is rich in phenolic compounds, antioxidant molecules. So, in this study it was determined the antioxidant potential of different fractions of leaves of Schinus terebinthifolius to select the fraction with the best antioxidant performance. For this purpose, Total reactive antioxidant potential (TRAP), DPPH and hydroxyl scavenging activities of hexane (HeF), dichloromethane (DcF), ethyl acetate (EtF) and methanol (MeF) fractions were measured. It was also performed a qualitative screening of these fractions, by thin layer chromatography (TLC) and the measure of total phenolic content. After, the selected fraction, MeF was diluted in saline and administered (20 mg / kg, i.p., 10 days) in rats with peripheral injury by chronic constriction injury (CCI), a model of neuropathic pain, and tissue injury by isolation of this nerve (sham), to evaluate their effects on mechanical and thermal sensitivity, and on the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione transferase (GST), and the concentrations of ascorbic acid (AA), total thiols (T-SH), hydrogen peroxide and lipid hydroperoxide (LPO) in the lumbosacral spinal cord and the cerebral cortex. Still, we evaluated possible effects of this fraction on locomotor and anxiety-like behaviors (open field and elevated plus maze tests), and on the enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), -glutamyl transpeptidase (GGT), birrubin and on body weight in naïve rats. CCI significantly induced thermal hyperalgesia and mechanical allodynia in animals treated with saline. The administration of MeF prevented the development of thermal hyperalgesia and increased mechanical threshold. Different oxidative stress parameters were altered in spinal cord and cerebral cortex of animals with sham CCI surgery, and the administration of MeF was able to modify some of these parameters, particularly in sham at three days. No significant differences were observed in locomotor and anxiety-like behaviors, plasma parameters and body weight. These results demonstrate that the MeF of Schinus terebinthifolius has antinociceptive properties and is able to change parameters of oxidative stress in the CNS in sham and CCI animals. Possibly these and other related anti-inflammatory activity, already described for components of Schinus terebinthifolius, are contributing to the antinociceptive fraction mechanisms. So, considering this potential and the absence of side effects related to the parameters studied, MeF may be a candidate for the treatment for painful conditions. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.lume.ufrgs.br/bitstream/handle/10183/101642/000933176.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://lume.ufrgs.br/bitstream/handle/10183/101642/000933176.pdf?sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
