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Simultaneous detection and identification of four sugarcane viruses by one-step RT-PCR.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xie, Yujia Wang, Mingqiang Xu, Donglin Li, Ruhui Zhou, Guohui |
| Copyright Year | 2009 |
| Abstract | Sugarcane mosaic disease (SMD) caused by the Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV) and Sugarcane streak mosaic virus (SCSMV) and sugarcane yellow leaf disease (SYLD) caused by the Sugarcane yellow leaf virus (SCYLV) are the two most prevalent and economically important viral diseases of sugarcane. In this study, a one-step quadruplex reverse transcription (RT)-PCR method that employed virus-specific primers was developed for the simultaneous detection and differentiation of SCMV, SrMV, SCSMV and SCYLV. Several sets of primers for each target virus were evaluated for their sensitivity and specificity by simplex and quadruplex RT-PCR. The optimum primer combinations and concentrations, RT temperature and time, and PCR annealing temperature and extension time were determined for the quadruplex RT-PCR. The assay was then validated using sugarcane samples affected with SMD and/or SYLD collected from sugarcane breeding fields and farmers' fields in southern China. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.jviromet.2009.07.015 |
| PubMed reference number | 19646484 |
| Journal | Medline |
| Volume Number | 162 |
| Issue Number | 1-2 |
| Alternate Webpage(s) | https://naldc.nal.usda.gov/download/36395/PDF |
| Alternate Webpage(s) | https://doi.org/10.1016/j.jviromet.2009.07.015 |
| Journal | Journal of virological methods |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
