Striking conservation of TFIID in Schizosaccharomyces pombe and Saccharomyces cerevisiae

Content Provider	Semantic Scholar
Author	Fikes, J. D. Becker, Daniel M. Winston, Fred Guarente, Leonard P.
Copyright Year	1990
Abstract	EUKARYOTIC promoters contain binding sites for basic transcription factors and gene-specific activator proteins1–6. The transcription factors interact at the TATA box, which lies close to the position of transcription initiation. Activators typically bind to distant sites that can lie kilobases away from the initiation site. The factor TFIID binds specifically to the TATA box to initiate an ordered pathway of assembly of the basic transcription factors5,7. Biochemical analyses have shown that human and Saccharomyces cerevisiae TFIID are functionally interchangeable in vitro8–10. To study further the functional conservation of this critical factor, we are surveying proteins from divergent organisms that can substitute in vivo for the S. cerevisiae TFIID. We report here the isolation of a unique gene from Schizosaccharomyces pombe that fully complements a null mutation in SPT15, the gene that encodes TFIID11–15 in S. cerevisiae. The Schiz. pombe gene encodes a protein 93% identical (166/178) to S. cerevisiae TFIID in a region consisting of a direct repeat.
Starting Page	291
Ending Page	294
Page Count	4
File Format	PDF HTM / HTML
DOI	10.1038/346291a0
PubMed reference number	2197558
Journal	Medline
Volume Number	346
Alternate Webpage(s)	http://genepath.med.harvard.edu/~winston/assets/22)-fikes-1990.pdf
Alternate Webpage(s)	https://doi.org/10.1038/346291a0
Journal	Nature
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article