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Metabolomics analysis of mangosteen (Garcinia mangostana Linn.) fruit pericarp using different extraction methods and GC-MS
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mamat, Siti Farah Azizan, Kamalrul Azlan Bin Baharum, Syarul Nataqain Noor, Normah Mohd Aizat, Wan Mohd |
| Copyright Year | 2018 |
| Abstract | Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32%), alcohols (9.59%), sugar acids (8.22%), organic acids (6.85%), phenolic acids (5.48%), aromatic compounds (2.74%) and aldehyde (1.37%). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit. |
| Starting Page | 89 |
| Ending Page | 97 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.21475/poj.11.02.18.pne1191 |
| Volume Number | 11 |
| Alternate Webpage(s) | http://www.pomics.com/mamat_11_2_2018_89_97.pdf |
| Alternate Webpage(s) | http://www.pomics.com/mamat_supp_11_2_2018_89_97.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
