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Purification of arachidonic acid from fungal single-cell oil via Al2O3-supported CuSO4 column chromatography
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Yunxiao Tao, Jing-Chao Wang, Lidong |
| Copyright Year | 2006 |
| Abstract | A new method has been proven successful to obtain high-purity arachidonic acid (AA), an important human nutrient, from fungal single-cell oil via Al2O3-supported CuSO4 column chromatography; the stationary phase is stable and readily reusable. In the first step, the mixed FA extracted from saponified fungal single-cell oil, containing 47.3% AA, were purified via urea inclusion to afford a fraction of PUFA containing 74.9% AA with an 85.9% yield. The enriched AA fraction was subsequently passed through an Al2O3-supported CuSO4 column with a hexane/acetone eluent to provide AA with 90.8% purity at a 46.5% yield. The total yield for the two-step process was 39.9%. |
| Starting Page | 659 |
| Ending Page | 662 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11746-006-1254-3 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s11746-006-1254-3 |
| Alternate Webpage(s) | https://doi.org/10.1007/s11746-006-1254-3 |
| Volume Number | 83 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |