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Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Kegui Song, Lei Dong, Xiuzhu |
| Copyright Year | 2010 |
| Abstract | Two strictly anaerobic, proteolytic bacterial strains, designated strain D3RC-2(T) and D3RC-3r, were isolated from a cellulose-degrading mixed culture enriched from yak rumen content. The strains were Gram-stain negative and non-spore-forming with cell sizes of 0.5-0.8 x 0.6-2.0 mum. The temperature range for growth was 24-46 degrees C (optimum 38-39 degrees C) and the pH range was between 5.6 and 8.7 (optimum 7.0-7.3). Both strains used soya peptone, tryptone, l-phenylalanine, l-leucine, l-methionine, l-serine, l-valine, l-threonine and l-histidine as carbon and nitrogen sources, but did not use any of the saccharides tested. The major fermentation products from PY medium were acetate, propionate and iso-butyrate. The DNA G+C contents of strains D3RC-2(T) and D3RC-3r were 41.0+/-0.1 mol% and 41.3+/-0.1 mol% (HPLC), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represented a new phyletic sublineage within the family Clostridiaceae, with <93.8 % 16S rRNA gene sequence similarity to recognized species. On the basis of the phenotypic, genotypic and physiological evidence, strains D3RC-2(T) and D3RC-3r are proposed as representing a novel species of a new genus, for which the name Proteiniclasticum ruminis gen. nov., sp. nov. is proposed. The type strain of the type species is D3RC-2(T) (=AS 1.5057(T)=JCM 14817(T)). |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/ijs.0.011759-0 |
| PubMed reference number | 19915115 |
| Journal | Medline |
| Volume Number | 60 |
| Part | 9 |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/ijsem/60/9/2221.pdf?accname=guest&checksum=5087D40268F9E3E0FAF04A2B734C425A&expires=1547017855&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/60/9/2221.pdf?accname=guest&checksum=20E20CE6428C3E8FA1398DF34910AB44&expires=1542937308&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/60/9/2221.pdf?accname=guest&checksum=FECF1AB7269E35CD3850B3B1610ACD99&expires=1544926735&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/ijsem/60/9/2221.pdf?accname=guest&checksum=CD0E5E2C90DC31ED7674801A5C91563C&expires=1550868287&id=id |
| Journal | International journal of systematic and evolutionary microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
