NDLI: Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2). Regulation by phosphatidylinositol 4, 5-bisphosphate.

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Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2). Regulation by phosphatidylinositol 4, 5-bisphosphate.

Content Provider	Semantic Scholar
Author	Heiska, Leena Alfthan, Kaija Grönholm, Mikaela Vilja, Pekka Vaheri, Antti Carpén, Olli
Copyright Year	1998
Abstract	Ezrin is a cytoplasmic linker molecule between plasma membrane components and the actin-containing cytoskeleton. We studied whether ezrin is associated with intercellular adhesion molecule (ICAM)-1, -2, and -3. In transfected cells, ICAM-1 and ICAM-2 colocalized with ezrin in microvillar projections, whereas an ICAM-1 construct attached to cell membrane via a glycophosphatidylinositol anchor was uniformly distributed on the cell surface. An interaction of ICAM-2 and ezrin was seen by affinity precipitation, microtiter binding assay, coimmunoprecipitation, and surface plasmon resonance methods. The calculated KD value was 3.3 x 10(-7) M. Phosphatidylinositol 4, 5-bisphosphate (PtdIns(4,5)P2) induced an interaction of ezrin and ICAM-1 and enhanced the interaction of ezrin and ICAM-2, but ICAM-3 did not bind ezrin even in the presence of PtdIns(4,5)P2. PtdIns(4, 5)P2 was shown to bind to cytoplasmic tails of ICAM-1 and ICAM-2, which are the first adhesion proteins demonstrated to interact with PtdIns(4,5)P2. The results indicate an interaction of ezrin with ICAM-1 and ICAM-2 and suggest a regulatory role of phosphoinositide signaling pathways in regulation of ICAM-ezrin interaction.
Starting Page	615
Ending Page	615
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jbc.org/content/273/34/21893.full.pdf
PubMed reference number	9705328v1
Volume Number	273
Issue Number	34
Journal	The Journal of biological chemistry
Language	English
Access Restriction	Open
Subject Keyword	Affinity Cell Adhesion Molecules Cell surface Cytoskeleton EZR gene Glycophosphatidylinositol ICAM3 protein, human Intercellular Adhesion Molecule 2 Intercellular Adhesion Molecules Intercellular adhesion molecule 1 Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Plasma membrane Projections and Predictions Surface Plasmon Resonance ezrin
Content Type	Text
Resource Type	Article

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