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Expression of pro-Muclin in pancreatic AR42J cells induces functional regulated secretory granules.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lisle, Robert C. De Norkina, Oxana Roach, Eileen A. Ziemer, Donna |
| Copyright Year | 2005 |
| Abstract | It is not clear how protein cargo is sorted to and retained in forming regulated secretory granules (RSG). Here, the sulfated mucin-type glycoprotein pro-Muclin was tested for its ability to induce RSG in the poorly differentiated rat pancreatic cell line AR42J. AR42J cells express RSG content proteins, but they fail to make granules. Adenovirus-pro-Muclin-infected AR42J cells store amylase, accumulate RSG, and respond to hormonal stimulation by secreting the stored protein. Expression of pro-Muclin combined with the inducing effect of dexamethasone resulted in a significant enhancement of the efficiency of regulated secretion. The effect of pro-Muclin was a strong decrease in constitutive secretion compared with dexamethasone-induction alone. A pro-Muclin construct missing the cytosolic tail domain was less effective at improving the efficiency of regulated secretion compared with the full-length construct. Increased expression of cargo (using adenovirus amylase) also modestly enhanced regulated secretion, indicating that part of pro-Muclin's effect may be due to increased expression of cargo protein. Overall, the data show that pro-Muclin acts as a sorting receptor that can induce RSG, and that its cytosolic tail is important in this process. |
| Starting Page | 495 |
| Ending Page | 501 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpcell.physiology.org/content/ajpcell/289/5/c1169.full.pdf |
| PubMed reference number | 15987769v1 |
| Volume Number | 289 |
| Issue Number | 5 |
| Journal | American journal of physiology. Cell physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amylases Cell secretion DMBT1 gene Dexamethasone Granule Mucins Secretory Vesicles |
| Content Type | Text |
| Resource Type | Article |
