Loading...
Please wait, while we are loading the content...
Similar Documents
Mechanisms of mitochondria-neurofilament interactions.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wagner, Oliver Ingvar Lifshitz, Jonathan Janmey, Paul. A. Linden, Marion Mcintosh, T. K. Leterrier, J. -F. |
| Copyright Year | 2003 |
| Abstract | Mitochondria are localized to regions of the cell where ATP consumption is high and are dispersed according to changes in local energy needs. In addition to motion directed by molecular motors, mitochondrial distribution in neuronal cells appears to depend on the docking of mitochondria to microtubules and neurofilaments. We examined interactions between mitochondria and neurofilaments using fluorescence microscopy, dynamic light scattering, atomic force microscopy, and sedimentation assays. Mitochondria-neurofilament interactions depend on mitochondrial membrane potential, as revealed by staining with a membrane potential sensitive dye (JC-1) in the presence of substrates/ADP or uncouplers (valinomycin/carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone) and are affected by the phosphorylation status of neurofilaments and neurofilament sidearms. Antibodies against the neurofilament heavy subunit disrupt binding between mitochondria and neurofilaments, and isolated neurofilament sidearms alone interact with mitochondria, suggesting that they mediate the interactions between the two structures. These data suggest that specific and regulated mitochondrial-neurofilament interactions occur in situ and may contribute to the dynamic distribution of these organelles within the cytoplasm of neurons. |
| Starting Page | 337 |
| Ending Page | 341 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jneurosci.org/content/jneuro/23/27/9046.full.pdf |
| Alternate Webpage(s) | http://www.s.kanazawa-u.ac.jp/phys/biophys/References/Mitochondria/9046.full.pdf |
| PubMed reference number | 14534238v1 |
| Volume Number | 23 |
| Issue Number | 27 |
| Journal | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenosine Diphosphate Adenosine Triphosphate Cyanides Dyes Membrane Potential, Mitochondrial Membrane Potentials Metabolic Process, Cellular Microscopy, Atomic Force Microscopy, Fluorescence Mitochondria Mitochondrial Membranes Neurofilaments Organelles Photon Correlation Spectroscopy Sedimentation process Staining method Valinomycin mitochondrion localization |
| Content Type | Text |
| Resource Type | Article |
