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The suppression of Arabidopsis succinic semialdehyde dehydrogenase (SSADH) phenotype by using ethyl methane-sulfonate mutagenesis (EMS)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dereje, W. Mekonnen Frank, Ludewig |
| Copyright Year | 2017 |
| Abstract | The disruption of the succinic semialdehyde dehydrogenase (SSADH) function in Arabidopsis leads to aberrant growth phenotype. In human, the SSADH malfunction leads to a condition known as γhydroxybutyrate (GHB) aciduria. It is characterized by the accumulation of GHB as well as abnormal growth. GHB aciduria is treated pharmacologically with compounds analogous to gamma-aminubutyrate (GABA). One of these compounds called vagabatrin effectively suppressed the ssadh phenotype in Arabidopsis. Furthermore, the Arabidopsis ssadh phenotype has been suppressed genetically by simultaneous mutation in the POP2 gene. In the present study, the presence of alternative solutions for suppression of the ssadh phenotype was investigated. For that, four ethyl methanesulfonate treated ssadh lines namely 7D, 13J, 17J and 21H were characterized phenotypically and chemotypically. Threeweek-old plants of the suppressor lines grew much better than the parent ssadh line and resembled the wild type. The suppressor lines also accumulated reduced amounts of GABA and GHB. PCR based mapping using Sequence Characterized Amplified Region (SCAR) markers located the mutations to chromosome 2 and chromosome 5, suggesting the involvement of at least two mutations in suppression of the ssadh phenotype. Deep whole-genome sequencing of line 17J identified several mutations that induced amino acid change, frame shift and a stop codon. The findings showed that there are more possibilities to suppress the ssadh phenotype; furthermore, it provides an opportunity to identify genes that interact with the GABA shunt. |
| Starting Page | 298 |
| Ending Page | 307 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.5897/AJPS2017.1552 |
| Volume Number | 11 |
| Alternate Webpage(s) | https://academicjournals.org/journal/AJPS/article-full-text-pdf/C7E985664756 |
| Alternate Webpage(s) | https://doi.org/10.5897/AJPS2017.1552 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
