NDLI: Solid phase DNA extraction on PDMS and direct amplification.

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Solid phase DNA extraction on PDMS and direct amplification.

Content Provider	Semantic Scholar
Author	Pasquardini, Laura Potrich, Cristina Quaglio, Marzia Lamberti, Andrea Guastella, Salvatore Antonio Lunelli, Lorenzo Cocuzza, Matteo Vanzetti, Lia Pirri, Candido F. Pederzolli, Cecilia
Copyright Year	2011
Abstract	In this paper we report an innovative use of Poly(DiMethyl)Siloxane (PDMS) to design a microfluidic device that combines, for the first time, in one single reaction chamber, DNA purification from a complex biological sample (blood) without elution and PCR without surface passivation agents. This result is achieved by exploiting the spontaneous chemical structure and nanomorphology of the material after casting. The observed surface organization leads to spontaneous DNA adsorption. This property allows on-chip complete protocols of purification of complex biological samples to be performed directly, starting from cells lysis. Amplification by PCR is performed directly on the adsorbed DNA, avoiding the elution process that is normally required by DNA purification protocols. The use of one single microfluidic volume for both DNA purification and amplification dramatically simplifies the structure of microfluidic devices for DNA preparation. X-Ray Photoelectron Spectroscopy (XPS) was used to analyze the surface chemical composition. Atomic Force Microscopy (AFM) and Field Emission Scanning Electron Microscopy (FESEM) were employed to assess the morphological nanostructure of the PDMS-chips. A confocal fluorescence analysis was utilized to check DNA distribution inside the chip.
Starting Page	347
Ending Page	355
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.rsc.org/suppdata/lc/c1/c1lc20371a/c1lc20371a.pdf
PubMed reference number	21989780v1
Alternate Webpage(s)	https://doi.org/10.1039/c1lc20371a
DOI	10.1039/c1lc20371a
Journal	Lab on a chip
Volume Number	11
Issue Number	23
Language	English
Access Restriction	Open
Subject Keyword	Chemical Structure Fluorescence Gene Amplification Technique Microchip Analytical Devices Microfluidics Nanostructured Materials Protocols documentation Scanning Electron Microscopy Transmission Electron Microscopy X-Ray Photoelectron Spectroscopy pathologic cytolysis
Content Type	Text
Resource Type	Article

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