NDLI: Determination of quinolones by high performance liquid chromatography using a monolitic reversed-phase column

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Determination of quinolones by high performance liquid chromatography using a monolitic reversed-phase column

Content Provider	Semantic Scholar
Author	Markman, Blanca Elena Ortega Koschtschak, Maria Regina Walter Yano, Helena Miyoco
Copyright Year	2005
Abstract	Quinolones are synthetic antibacterial agents used for treating a wide variety of infections of urinary tract, gastrointestinal tract, and skin. The aim of this study was to develop a method for performing the determination of ciprofloxacin, ciprofloxacin ethylenediamine analog (a related compound), and norfloxacin by means of high performance liquid chromatographic (HPLC) technique. The compounds were separated on a Chromolith RP18 column, with isocratic system. The mobile phase was a mixture of phosphoric acid 0.042 mol/L, pH 3.0 ± 0.1: acetonitrile (87:13), flow rate of 1.6 mL/min and UV detection at 275nm. The range of calibration curve was 20.0–125.0 μg/mL for ciprofloxacin and 20.0–100.0 μg/mL for norfloxacin. The correlation coefficient was 0.9999 for ciprofloxacin and 0.9999 for norfloxacin. The retention time for ciprofloxacin was 3.4 min, for ethilenodiamine ciprofloxacin was 2.4 min, and 2.9 min for norfloxacin. Resolution between ciprofloxacin and ciprofloxacin ethylenediamine analog was 1.1. The proposed HPLC method is rapid, simple, accurate, reproducible, and it can be applied in analyzing raw-materials, as well as in determining ciprofloxacin, ciprofloxacin ethylenediamine and norfloxacin in diverse formats as capsules, tablets, and in solution for infusion.
Starting Page	65
Ending Page	69
Page Count	5
File Format	PDF HTM / HTML
Volume Number	64
Alternate Webpage(s)	http://periodicos.ses.sp.bvs.br/pdf/rial/v64n1/v64n1a10.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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