NDLI: Radiation-stimulated Dna Synthesis in Cultured Mammalian Cells

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Radiation-stimulated Dna Synthesis in Cultured Mammalian Cells

Content Provider	Semantic Scholar
Author	Rasmussen, Ronald E. Painter, Robert B.
Copyright Year	1966
Abstract	A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H(3)-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G(1) and G(2) cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.
Starting Page	11
Ending Page	19
Page Count	9
File Format	PDF HTM / HTML
PubMed reference number	5920187v1
Volume Number	29
Journal	The Journal of cell biology
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/e9/11.PMC2106960.pdf
Language	English
Access Restriction	Open
Subject Keyword	Autoradiography Bromodeoxyuridine Bromouracil Centrifugation Centrifugation, Density Gradient Cultured Cell Line Cultured Cells Mammals Ultraviolet Rays
Content Type	Text
Resource Type	Article

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