Inflammatory mediators release calcitonin gene-related peptide from dorsal root ganglion neurons of the rat

Content Provider	Semantic Scholar
Author	Averbeck, Beate Izydorczyk, Iwona Kress, Michaela
Copyright Year	2000
Abstract	The interactions between the inflammatory mediators bradykinin, serotonin, prostaglandin E(2) and acid pH were studied in rat dorsal root ganglion neurons in culture. For this purpose, the cultures were stimulated by inflammatory mediators (bradykinin, serotonin, prostaglandin E(2), 10(-5)M each) or acid solution (pH 6.1) for 5 min and the content of calcitonin gene-related peptide was determined in the supernatant before, during and after stimulation, using an enzyme immunoassay. Acid solution resulted in a threefold increase of the basal calcitonin gene-related peptide release which was entirely dependent on the presence of extracellular calcium. The release could not be blocked by the addition of the capsaicin antagonist capsazepine (10(-5)M). Bradykinin (10(-5)M) caused a 50% increase of the basal calcitonin gene-related peptide release which was again dependent on the presence of extracellular calcium, whereas serotonin and prostaglandin E(2) were each ineffective at 10(-5)M concentration. The combination of bradykinin, serotonin and prostaglandin E(2) led to a fivefold increase of the calcitonin gene-related peptide release which could not be further enhanced by acidification. The competitive capsaicin receptor antagonist capsazepine (10(-5)M) significantly reduced the release induced by the combination of bradykinin, serotonin and prostaglandin E(2). It is suggested that the inflammatory mediators co-operate and together may act as endogenous agonists at the capsaicin receptor to cause calcium influx and consecutive neuropeptide release.
Starting Page	135
Ending Page	140
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1016/S0306-4522(00)00095-6
PubMed reference number	10858619
Journal	Medline
Volume Number	98
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0306452200000956
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0306452200000956?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/S0306-4522%2800%2900095-6
Journal	Neuroscience
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article