NDLI: A biodegradable polyurethane-ascorbic acid scaffold for bone tissue engineering.

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A biodegradable polyurethane-ascorbic acid scaffold for bone tissue engineering.

Content Provider	Semantic Scholar
Author	Zhang, Jianying Doll, Bruce A. Beckman, Eric J. Hollinger, Jeffrey O.
Copyright Year	2003
Abstract	A novel, nontoxic, biodegradable, sponge-like polyurethane scaffold was synthesized from lysine-di-isocyanate (LDI) and glycerol. Ascorbic acid (AA) was copolymerized with LDI-glycerol. Our hypothesis was that the AA-containing polymer foam would enhance the biological activity of the osteoblastic precursor cell (OPCs). The LDI-glycerol-AA matrix degraded in aqueous solution to the nontoxic products of lysine, glycerol, and AA. The degradation products did not significantly affect the solution pH. The physical properties of the polymer network supported the cell growth in vitro. Mouse OPCs attached to the polymer matrix and remained viable. OPCs produced multilayered confluent cultures, a characteristic typical of bone cells. Furthermore, AA release stimulated cell proliferation, type I collagen, and alkaline phosphatase synthesis. Cells grown on the LDI-glycerol-AA matrix also showed an enhancement of mRNA expression for pro-alpha1 (I) collagen and transforming growth factor-alpha1 after 1 week. Data were tested for significance with an analysis of variance model and multiple comparison test (Fisher's Protected Least Significant Difference) at p < or = 0.05. The observations suggest that AA-containing polyurethane may be useful in bone tissue engineering applications.
Starting Page	389
Ending Page	400
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1002/jbm.a.10015
Alternate Webpage(s)	http://pdf-s3.xuebalib.com:1262/1k9jW4MEGRPY.pdf?down
PubMed reference number	14566779
Alternate Webpage(s)	https://doi.org/10.1002/jbm.a.10015
Journal	Medline
Volume Number	67
Issue Number	2
Journal	Journal of biomedical materials research. Part A
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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