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Synthesis of pyruvate: ferredoxin oxidoreductase and alcohol dehydrogenase E enzymes during Giardia intestinalis excystation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Niño, Carlos Alberto Wasserman, Moisés M. |
| Copyright Year | 2010 |
| Abstract | INTRODUCTION Giardia intestinalis is a unicellular parasite of worldwide distribution. It causes an intestinal illness known as giardiasis, and it is probably the earliest diverging eukaryotic microorganism. Previously, changes have been reported in the expression of mRNAs at several stages of the life cycle; however specific enzymatic activity changes have not been explored. OBJECTIVE The expression of pyruvate ferredoxin oxidoreductase (PFOR) and alcohol dehydrogenase E (ADHE) enzymes was measured in cyst and trophozoite stages, and during the excystation process. MATERIALS AND METHODS Recombinant proteins were generated for PFOR and ADHE to be used as antigens in the production of polyclonal antibodies for the detection of native proteins by Western Blot. The enzymatic activity of ADHE and glutamate dehydrogenase (GDH) was evaluated by spectrophotometric assays. RESULTS PFOR (139 kDa) and ADHE (97 kDa) proteins were detected in trophozoites, but not in cysts. During excystation, ADHE protein was detected after the first phase of induction, but the PFOR protein appeared only after the second phase. This indicated that both proteins were synthesized during excystation, although at different times. ADHE enzymatic activity was present only in trophozoites and not in cysts whereas GDH activity was detected in both stages. CONCLUSION These results conclusively showed that PFOR and ADHE enzymes were translated during the excystation process and is strong evidence that active protein synthesis was occurring during excystation. |
| Starting Page | 1 |
| Ending Page | 3 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.redalyc.org/pdf/843/84312378005.pdf |
| Alternate Webpage(s) | http://www.scielo.org.co/pdf/bio/v30n1/v30n1a05.pdf |
| PubMed reference number | 20890547v1 |
| Volume Number | 30 |
| Issue Number | 1 |
| Journal | Biomedica : revista del Instituto Nacional de Salud |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alcohol dehydrogenase CDISC SDTM Microorganism Terminology Cyst Ethanol 0.62 ML/ML Topical Gel Ferredoxin Giardia lamblia Ab:Titr:Pt:Ser:Qn Giardiasis Glutamate Dehydrogenase Glutamic Acid Pyruvates Trophozoite Western Blotting excystation pyruvate synthase activity |
| Content Type | Text |
| Resource Type | Article |
