NDLI: Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.

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Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.

Content Provider	Semantic Scholar
Author	Feng, Liang Senchenkova, Sof'ya N. Tao, Jiang Shashkov, A. S. J. H. U. Liu, Bin Shevelev, Sergei D. Reeves, P. R. Xu, Jian-Guo Knirel, Yuriy A. Wang, Lei
Copyright Year	2005
Abstract	Enterohemorrhagic Escherichia coli O145 strains are emerging as causes of hemorrhagic colitis and hemolytic uremic syndrome. In this study, we present the structure of the E. coli O145 O antigen and the sequence of its gene cluster. The O145 antigen has repeat units containing three monosaccharide residues: 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamidoylamino-2,6-dideoxy-L-galactose, and N-acetylneuraminic acid. It is very closely related to Salmonella enterica serovar Touera and S. enterica subsp. arizonae O21 antigen. The E. coli O145 gene cluster is located between the JUMPStart sequence and the gnd gene and consists of 15 open reading frames. Putative genes for the synthesis of the O-antigen constituents, for sugar transferase, and for O-antigen processing were annotated based on sequence similarities and the presence of conserved regions. The putative genes located in the E. coli O145 O-antigen gene cluster accounted for all functions expected for synthesis of the structure. An E. coli O145 serogroup-specific PCR assay based on the genes wzx and wzy was also developed by screening E. coli and Shigella isolates of different serotypes.
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Ending Page	A
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.tjbiochip.com/cn/pages/technology/doc/34.pdf
PubMed reference number	15629947v1
Volume Number	187
Issue Number	2
Journal	Journal of bacteriology
Language	English
Access Restriction	Open
Subject Keyword	Antigen Processing Enterohemorrhagic Escherichia coli Frame (physical object) Galactose Hemolytic-Uremic Syndrome Impaired glucose tolerance Monosaccharides O Antigens Open Reading Frames Reading Frames (Nucleotide Sequence) Salmonella enterica subsp. enterica serovar enteritidis Serogroup Serotype Subspecies Sugars Thioctic Acid Transferase hemorrhagic colitis
Content Type	Text
Resource Type	Article

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