NDLI: A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus

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A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus

Content Provider	Semantic Scholar
Author	Nakano, Aiko Muramatsu, Masaaki
Copyright Year	1989
Abstract	SAR1, a gene that has been isolated as a multicopy suppressor of the yeast ER-Golgi transport mutant sec12, encodes a novel GTP-binding protein. Its nucleotide sequence predicts a 21-kD polypeptide that contains amino acid sequences highly homologous to GTP-binding domains of many ras-related proteins. Gene disruption experiments show that SAR1 is essential for cell growth. To test its function further, SAR1 has been placed under control of the GAL1 promoter and introduced into a haploid cell that had its chromosomal SAR1 copy disrupted. This mutant grows normally in galactose medium but arrests growth 12-15 h after transfer to glucose medium. At the same time, mutant cells accumulate ER precursor forms of a secretory pheromone, alpha-mating factor, and a vacuolar enzyme, carboxypeptidase Y. We propose that Sec12p and Sarlp collaborate in directing ER-Golgi protein transport.
Starting Page	2677
Ending Page	2691
Page Count	15
File Format	PDF HTM / HTML
PubMed reference number	2512296
Volume Number	109
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/109/6/2677.full.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/109/6/2677.full.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/67/a7/jc10962677.PMC2115904.pdf
Language	English
Access Restriction	Open
Subject Keyword	Amino Acid Sequence Amino Acids Base Sequence Cell secretion ER to Golgi vesicle-mediated transport Endoplasmic Reticulum F Factor Galactose Genes, vif Glucose Guanosine Triphosphate Haploidy IQGAP1 gene Nucleotides Pheromone Polypeptides SAR1A gene Vacuole cell growth intracellular protein transport ras Oncogene
Content Type	Text
Resource Type	Article

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