NDLI: Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.

Content Provider	Semantic Scholar
Author	Sonenberg, Nahum Rupprecht, Kathleen M. Hecht, Sidney M. Shatkin, A. J.
Copyright Year	1979
Abstract	A 24,000-dalton polypeptide that binds strongly and can be specifically crosslinked to the 5'-terminal cap structure m7GpppN in eukaryotic mRNAs has been detected in protein synthesis initiation factor preparations [Proc. Natl. Acad. Sci. USA (1978) 75, 4843--4847]. This polypeptide has been purified to apparent homogeneity by one chromatographic passage through an affinity resin prepared by coupling the levulinic acid O2',3'-acetal of m7GDP to AH-Sepharose 4B. Translation, in HeLa cell extracts, of capped mRNAs including Sindbis virus, reovirus, and rabbit globin mRNAs was stimulated by the cap-binding protein under conditions that did not increase translation of noncapped RNAs of encephalomyocarditis virus and satellite tobacco necrosis virus.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.76.9.4345
PubMed reference number	291969
Journal	Medline
Volume Number	76
Issue Number	9
Alternate Webpage(s)	http://europepmc.org/articles/pmc411571?pdf=render
Alternate Webpage(s)	https://doi.org/10.1073/pnas.76.9.4345
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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