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VCAM-1 expression on dystrophic muscle vessels has a critical role in the recruitment of human blood-derived CD133+ stem cells after intra-arterial transplantation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gavina, Manuela Belicchi, Marzia Rossi, Barbara A. Ottoboni, Linda Colombo, Fabio Meregalli, Mirella Battistelli, Maurizio Forzenigo, Laura Virginia Biondetti, Piero Pisati, Federica Parolini, Daniele Farini, Andrea Issekutz, Andrew C. Bresolin, N. Rustichelli, Franco Constantin, Gabriela Torrente, Yvan |
| Copyright Year | 2006 |
| Abstract | Recently our group demonstrated the myogenic capacity of human CD133(+) cells isolated from peripheral blood when delivered in vivo through the arterial circulation into the muscle of dystrophic scid/mdx mice. CD133(+) stem cells express the adhesion molecules CD44, LFA-1, PSGL-1, alpha4-integrins, L-selectin, and chemokine receptor CCR7. Moreover these cells adhere in vitro to VCAM-1 spontaneously and after stimulation with CCL19. Importantly, after muscle exercise, we found that the expression of VCAM-1 is strongly up-regulated in dystrophic muscle vessels, whereas the number of rolling and firmly adhered CD133(+) stem cells significantly increased. Moreover, human dystrophin expression was significantly increased when muscle exercise was performed 24 hours before the intra-arterial injection of human CD133(+) cells. Finally, treatment of exercised dystrophic mice with anti-VCAM-1 antibodies led to a dramatic blockade of CD133(+) stem cell migration into the dystrophic muscle. Our results show for the first time that the expression of VCAM-1 on dystrophic muscle vessels induced by exercise controls muscle homing of human CD133(+) stem cells, opening new perspectives for a potential therapy of muscular dystrophy based on the intra-arterial delivery of CD133(+) stem cells. |
| File Format | PDF HTM / HTML |
| DOI | 10.1182/blood-2006-04-018564 |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/108/8/2857.full.pdf?sso-checked=true |
| Alternate Webpage(s) | http://www.formazione.eu.com/_documents/progetti/uploads/pr_produzione/DOCUMENTO395.pdf |
| PubMed reference number | 16809613 |
| Alternate Webpage(s) | https://doi.org/10.1182/blood-2006-04-018564 |
| Journal | Medline |
| Volume Number | 108 |
| Issue Number | 8 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
