Fast and continuous plasma extraction from whole human blood based on expanding cell-free layer devices

Content Provider	Semantic Scholar
Author	Sollier, Elodie Cubizolles, Myriam Fouillet, Yves Achard, J.
Copyright Year	2010
Abstract	This paper presents promising microfluidic devices designed for continuous and passive extraction of plasma from whole human blood. These designs are based on red cells lateral migration and the resulting cell-free layer locally expanded by geometric singularities such as an enlargement of the channel or a cavity adjacent to the channel. After an explanation of flow patterns, different tests are described that confirm the advantages of both proposed singularities, providing a 1.5 and 2X increase in extraction yield compared to a reference device, for 1:20 diluted blood at 100 µL/min. Devices have also been successively optimized, with extraction yields up to 17.8%, and biologically validated for plasma extraction, with no protein loss or denaturation, no hemolysis and with excellent cell purity. Finally, the dilution effect has been experimentally investigated.
Starting Page	485
Ending Page	497
Page Count	13
File Format	PDF HTM / HTML
DOI	10.1007/s10544-010-9405-6
PubMed reference number	20204703
Journal	Medline
Volume Number	12
Alternate Webpage(s)	http://www.ipb.pt/~ruimec/index_ficheiros/FCTmbs/ref_11.pdf
Journal	Biomedical microdevices
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article