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Expression cloning of novel regulators of 92 kDa type IV collagenase expression.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nair, Rajesh Ramakrishnan Boyd, Douglas D. |
| Copyright Year | 2005 |
| Abstract | Overexpression of the 92 kDa type IV collagenase (MMP-9) contributes to cancer progression. However, to date, there are few known regulators of expression of this metalloproteinase. We employed an expression library comprising 500,000 cDNA clones to screen for novel regulators of MMP-9 expression. HT1080 cells were transiently co-transfected with an MMP-9 promoter-luciferase reporter and pools of the cDNA expression library. Positive-scoring pools were subdivided in secondary and tertiary screens, after which the regulatory cDNAs were identified by DNA sequencing. This brief review illustrates the utility of expression cloning in identifying specific regulators of MMP-9 expression. |
| Starting Page | 108P |
| Ending Page | 109P |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemsoctrans.org/bst/033/1135/0331135.pdf |
| PubMed reference number | 16246065v1 |
| Volume Number | 33 |
| Part | 5 |
| Journal | Biochemical Society transactions |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | COLLAGENASE Clone Cells DNA, Complementary Luciferases Metalloproteases Neoplasms negative regulation of type IV pilus biogenesis tertiary |
| Content Type | Text |
| Resource Type | Article |
