NDLI: Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

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Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

Content Provider	Semantic Scholar
Author	Park, Sung-Jin Choi, Jun-Hyeok Jung, Yeon-Seop Yu, Mi hee
Copyright Year	2013
Abstract	Sung-Jin Park, Jun-Hyeok Choi, Yeon-Seop Jung, and Mi Hee Yu*Department of Food Science and Technology, Keimyung UniversityAbstract The anti-obesity effect of ethanol extract and their fractions from Rumex Crispus L. on the differentiation of3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation withOil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibitedadipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fataccumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of anadipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin- along with ethyl acetate fraction residueprocessing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferators-activated-receptor-γ (PPARγ) and CCAAT enhancer-binding-proteins α (C/EBPα). These results indicate that ethyl acetatefraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be neededto identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.Keywords: Rumex Crispus L., adipocytes, PPAR γ, C/EBPα, SREBP1c
Starting Page	90
Ending Page	96
Page Count	7
File Format	PDF HTM / HTML
DOI	10.9721/KJFST.2013.45.1.90
Volume Number	45
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/ksfst1/SPGHB5/2013/v45n1/SPGHB5_2013_v45n1_90.pdf
Alternate Webpage(s)	https://doi.org/10.9721/KJFST.2013.45.1.90
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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