NDLI: Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology

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Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology

Content Provider	Semantic Scholar
Author	Zhu, Ruoqing Shevchenko, Olga Ma, Cathleen Maury, S. Freitag, Michael Strauss, Steven H.
Copyright Year	2013
Abstract	The Arabidopsis thaliana DDM1 (Decreased DNA Methylation) gene is necessary for the maintenance of DNA methylation and heterochromatin assembly. In Arabidopsis, ddm1 mutants exhibit strong but delayed morphological phenotypes. We used RNA interference (RNAi) to suppress transcripts of two orthologous DDM1 paralogs in Populus trichocarpa and examined effects on whole plant phenotypes during perennial growth and seasonal dormancy. The RNAi-PtDDM1 transgenic poplars showed a wide range of DDM1 transcript suppression; the most strongly suppressed line had 37.5 % of the expression of the non-transgenic control. Genomic cytosine methylation (mC %) was 11.1 % in the non-transgenic control, compared with 9.1 % for the transgenic event with lowest mC %, a reduction of 18.1 %. An evaluation of greenhouse growth directly after acclimation of in vitro grown plants showed no developmental or growth rate abnormalities associated with the decrease in PtDDM1 expression. However, after a dormancy cycle and growth outdoors, a mottled leaf phenotype appeared in some of the transgenic insertion events that had strongly reduced PtDDM1 expression and DNA methylation. The phenotypic consequences of reduced DDM1 activity and DNA methylation appears to increase with cumulative plant propagation and growth.
Starting Page	1483
Ending Page	1493
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1007/s00425-013-1858-4
PubMed reference number	23455459
Journal	Medline
Volume Number	237
Alternate Webpage(s)	http://people.forestry.oregonstate.edu/steve-strauss/sites/people.forestry.oregonstate.edu.steve-strauss/files/Zhu_Planta2013.pdf
Journal	Planta
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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