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BCR-ABL-induced adhesion defects are tyrosine kinase-independent.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wertheim, Jason A. Forsythe, Kevin Druker, Brian J. Hammer, Daniel A. Boettiger, David Pear, Warren S. |
| Copyright Year | 2002 |
| Abstract | The t(9;22) chromosomal translocation results in expression of P210(BCR-ABL), a fusion protein necessary for the development of chronic myelogenous leukemia (CML). The constitutive activation of the P210(BCR-ABL) tyrosine kinase results in phosphorylation of multiple signaling pathways leading to the transformed phenotype. Additionally, extracellular interactions between P210(BCR-ABL)-expressing progenitor cells and bone marrow stroma may provide external signals that facilitate CML development. In contrast to the intracellular signaling pathways involved in CML, little is known about how P210(BCR-ABL) expression modifies cell-cell and cell-substratum interactions. To investigate the role of P210(BCR-ABL) in modulating cellular adhesion, we used a highly sensitive and quantitative cell detachment apparatus that measures the strength of association between a population of cells and an adhesive matrix. Our findings show that P210(BCR-ABL) expression increased adhesion nearly 2-fold between the myeloblastic cell line, 32D, and fibronectin compared to a control vector. We then investigated whether abnormal adhesion due to P210(BCR-ABL) expression was caused by its tyrosine kinase activity. A quantitative analysis of cell-fibronectin adhesion found that neither expression of a kinase-inactive P210(BCR-ABL) mutant in 32D cells or attenuation of kinase activity by STI571 (imatinib mesylate) in 32D cells transduced with wild-type P210(BCR-ABL) could correct the nearly 2-fold increase in cell-fibronectin adhesion. Similarly, STI571 treatment of Meg-01 cells, a P210(BCR-ABL)-expressing cell line derived from a patient in blast crisis, failed to inhibit adhesion to fibronectin. Together, our results indicate that changes in adhesion induced by P210(BCR-ABL) are independent of its tyrosine kinase activity. |
| Starting Page | 46 |
| Ending Page | 58 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/99/11/4122.full.pdf?sso-checked=true |
| PubMed reference number | 12010816v1 |
| Volume Number | 99 |
| Issue Number | 11 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adhesives Biospecimen Core Resource Facility Blast Phase Bone Marrow Cell Adhesion Chromosomal translocation Fusion Proteins, bcr-abl Imatinib mesylate Leukemia, B-Cell Mesylates Myeloid Leukemia Myeloid Leukemia, Chronic Patients Protein Tyrosine Kinase Stroma abl Oncogene myeloblast t(9;22) |
| Content Type | Text |
| Resource Type | Article |
