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A possible connection between adhesion regulating molecule 1 overexpression and nuclear factor kappa B activity in hepatocarcinogenesis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yang, Xiaodong Miao, Xiongying Wen, Yu Chih Hu, Jixiong Dai, Weidong Yin, Bangliang |
| Copyright Year | 2012 |
| Abstract | Adhesion regulating molecule 1 (ADRM1), a 19S proteasome cap-associated protein, and nuclear factor kappa B (NF-κB), a protein transcription factor controlling DNA transcription, may play an important role in tumorigenesis. Overexpression of ADRM1 and activation of NF-κB are well-observed in hepatocellular carcinoma (HCC). However, little is known about whether both are functionally connected during hepatocarcinogenesis, and the mechanisms involved. In this study, using laboratory techniques including short hairpin RNA (shRNA)-mediated knockdown, immunohistochemistry (IHC), both semi-quantitative and real-time RT-PCR, western blotting, MTT assay, transwell assay, flow cytometry and electrophoretic mobility shift assay (EMSA), the expression of ADRM1, the effects of ADRM1 knockdown on NF-κB activity, as well as the biological behavior of HCC cells including proliferation, migration, invasion and apoptosis were investigated in the samples from HCC patients and HCC cell lines. We found that both mRNA and protein levels of ADRM1 were increased in HCC tissues and that this increase in ADRM1 expression was parallel to the metastatic potential of HCC cell lines. After ADRM1 knockdown in MHCC97-H cells, the expression of IκB-α was increased and the NF-κB activity was reduced. Furthermore, ADRM1 knockdown inhibited MHCC97-H cell proliferation and induced cell apoptosis, and the migration and invasion of MHCC97-H cells were significantly repressed. These results indicate that there is a clear functional connection between ADRM1 and NF-κB in hepatocarcinogenesis, despite the precise mechanisms through which the two work together still being unknown. |
| Starting Page | 252 |
| Ending Page | 253 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/or/28/1/283/download |
| PubMed reference number | 22576803v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/or.2012.1767 |
| DOI | 10.3892/or.2012.1767 |
| Journal | Oncology reports |
| Volume Number | 28 |
| Issue Number | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ADRM1 gene Apoptosis Body tissue Carcinogenesis Cell Proliferation Cultured Cell Line Electrophoretic Mobility Shift Assay F Factor Flow Cytometry Hepatocarcinogenesis Immunohistochemistry Liver carcinoma NF-kappa B Patients Short Hairpin RNA Staphylococcal Protein A TRANSCRIPTION FACTOR Western Blotting monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
