NDLI: Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus

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Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus

Content Provider	Semantic Scholar
Author	Kado, Yuji Inoue, Tsuyoshi Ishikawa, Kazuhiko
Copyright Year	2011
Abstract	Three categories of cellulases, endoglucanases, cellobiohydrolases and β-glucosidases, are commonly used in the process of cellulose saccharification. In particular, the activity and characteristics of hyperthermophilic β-glucosidase make it promising in industrial applications of biomass. In this paper, the crystal structure of the hyperthermophilic β-glucosidase from Pyrococcus furiosus (BGLPf) was determined at 2.35 Å resolution in a new crystal form. The structure showed that there is one tetramer in the asymmetric unit and that the dimeric molecule exhibits a structure that is stable towards sodium dodecyl sulfate (SDS). The dimeric molecule migrated in reducing SDS polyacrylamide gel electrophoresis (SDS-PAGE) buffer even after boiling at 368 K. Energy calculations demonstrated that one of the two dimer interfaces acquired the largest solvation free energy. Structural comparison and sequence alignment with mesophilic β-glucosidase A from Clostridium cellulovorans (BGLACc) revealed that the elongation at the C-terminal end forms a hydrophobic patch at the dimer interface that might contribute to hyperthermostability.
Starting Page	1473
Ending Page	1479
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1107/S1744309111035238
PubMed reference number	22139147
Journal	Medline
Volume Number	67
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/d7/d0/f-67-01473.PMC3232120.pdf
Alternate Webpage(s)	http://journals.iucr.org/f/issues/2011/12/00/be5175/be5175.pdf
Alternate Webpage(s)	https://doi.org/10.1107/S1744309111035238
Journal	Acta crystallographica. Section F, Structural biology and crystallization communications
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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Structure of hyperthermophilic β-glucosidase from Pyrococcus furiosus.

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