Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors.

Content Provider	Semantic Scholar
Author	Foster, Leonard J. Hoog, Carmen L. De Mann, Matthias
Copyright Year	2003
Abstract	Membrane lipids were once thought to be homogenously distributed in the 2D surface of a membrane, but the lipid raft theory suggests that cholesterol and sphingolipids partition away from other membrane lipids. Lipid raft theory further implicates these cholesterol-rich domains in many processes such as signaling and vesicle traffic. However, direct characterization of rafts has been difficult, because they cannot be isolated in pure form. In the first functional proteomic analysis of rafts, we use quantitative high-resolution MS to specifically detect proteins depleted from rafts by cholesterol-disrupting drugs, resulting in a set of 241 authentic lipid raft components. We detect a large proportion of signaling molecules, highly enriched versus total membranes and detergent-resistant fractions, which thus far biochemically defined rafts. Our results provide the first large-scale and unbiased evidence, to our knowledge, for the connection of rafts with signaling and place limits on the fraction of plasma membrane composed by rafts.
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/100/10/5813.full.pdf
PubMed reference number	12724530v1
Volume Number	100
Issue Number	10
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Cholesterol Detergents Lipid Metabolism Disorders Membrane Lipids Plasma membrane Proteomics Signal Transduction Sphingolipids Tissue membrane Vesicle (morphologic abnormality) lipid raft
Content Type	Text
Resource Type	Article