Effect of recombinant IFN-gamma on igE-dependent leukotriene generation by peripheral blood leukocytes in patients with pollinosis and asthma.

Content Provider	Semantic Scholar
Author	Krasnowska, Maryla Mędrala, Wojciech Małolepszy, J. Krasnowski, Ryszard
Copyright Year	2000
Abstract	Interferon gamma (IFN-gamma) is considered one of the causative and intensifying factors in inflammation. The reaction to allergens releases IFN-gamma, an immunomodulatory cytokine known to inhibit IgE synthesis and Th cell proliferation. The aim of the study was to evaluate the influence of IFN-gamma on leukotriene (LT) release in vitro, from human leukocytes of atopic patients with pollinosis and asthma. Thirty-eight patients were enrolled in the study: 15 with pollinosis and 23 asthmatics. In the presence of IL-3, leukocytes were stimulated with specific allergens. Other samples of leukocytes were preincubated with different concentrations of IFN-gamma for 15 min before allergen stimulation. The concentration of LT in supernatants was measured according to the CAST-ELISA procedure. We stated that IFN-gamma had significantly diminished LT release in a dose-dependent mode from the leukocytes of pollinotics. IFN-gamma did not change LT release in the asthmatic group, although, in leukocytes the small and medium basic production of LT, IFN-gamma caused a statistically significant fall in LT generation.
File Format	PDF HTM / HTML
PubMed reference number	11059646
Journal	Medline
Volume Number	48
Issue Number	4
Alternate Webpage(s)	https://www.iitd.pan.wroc.pl/files/AITEFullText/48z409.pdf
Journal	Archivum immunologiae et therapiae experimentalis
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article