A versatile method of identifying speci W c binding proteins on a Y nity resins

Content Provider	Semantic Scholar
Author	Yamamoto, Kiyoshi Yamazaki, Akira Takeuchi, Mikio Tanaka, Akito
Copyright Year	2006
Abstract	The isolation of both speciWc and nonspeciWc binding proteins on aYnity matrices bearing bioactive compounds hinders the identiWcation of drug cellular targets. Although solid-phase elution and competition methods conventionally are used to distinguish between speciWc and nonspeciWc receptor–ligand interactions, these approaches often are severely restricted by low ligand solubility and/or slow kinetic dissociation. This article describes an alternative and versatile method, termed serial aYnity chromatography, to identify ligand receptors using aYnity resins. © 2006 Elsevier Inc. All rights reserved.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www2.huhs.ac.jp/~h070016a/AnalBiochem352(1)15(2006).pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article